Fig. 5

P/PB/N/R activated the adaptive immune response in tumors. A–D Representative flow cytometric analysis images and the relative quantification of M2-like macrophages (CD206hi) and M1-like macrophages (CD80hi) gating on F4/80 + CD11b + CD45 + cells. E–F) Representative flow cytometric analysis gating on CD3 + cells and absolute quantification of CD8 + and CD4 + T cells in the tumor. G, H Representative flow cytometric analysis images and relative quantification of CD4 + Foxp3 + T cells gating on CD3 + CD4 + cells. I, J Representative flow cytometric analysis images and relative quantification of CD103 + dendritic cells gating on CD45 + CD11c + cells. K, L Representative flow cytometric analysis images and relative quantification of NK1.1 + CD3- NK cells gating on CD45 + cells. Data are presented as the mean ± SEM (n = 3). Statistical significance was calculated via a two-tailed Student’s t test. *P < 0. 05; **P < 0. 01; ***P < 0.001; ****P < 0.0001