Fig. 5

HA-P5 reduced the sebum accumulation in SZ95 cells with the involvement of FGFRs and AR signalling pathways. A Oil red O staining indicates HA-P5 and AZD4547 reduced the sebum over-accumulation induced by DHT in SZ95 cells; B Quantification of TG in SZ95 cells treated with HA-P5 and AZD4547 with the presence of DHT. One-way ANOVA was employed for statistical analysis. ***p < 0.001, ****p < 0.0001; C Western blotting analyzes the key molecules of FGFRs and AR signalling pathways in response to the treatment of HA-P5 and AZD4547 with the presence of DHT; D qPCR quantified the expression of AKR1C3 in SZ95 cells treated with HA-P5 and AZD4547 with the presence of DHT. One-way ANOVA was employed for statistical analysis. Columns with different letters refer to p < 0.01; E Western blotting analyzes the protein level of AKR1C3 in SZ95 cells treated with HA-P5 and AZD4547 with the presence of DHT