Fig. 4
A Schematic diagram of mechanism of intracellular acidity enhancement. Determination of intracellular p-AMPK protein levels in B MCF-7 cells and C 4T1 cells by western blotting assay. Intracellular lactate content of D MCF-7 and E 4T1 cells incubated with PBS, FePt alloys, and LMOFePt-T at 5 μg/mL (equivalent Fe) for 12 h. F H2O2 content of MCF-7 cells treated with different samples at the concentration of 5 μg/mL (equivalent Fe) for 12 h. G CLSM observation for the acidity of MCF-7 cells stained by BCECF-AM after different samples at the concentration of 5 μg/mL (equivalent Fe) for 12 h. H GSH level of MCF-7 cells incubated with different concentrations of pLMOFePt-TGO. I GSH level of MCF-7 cells incubated with LMOFePt-TGO and pLMOFePt-TGO at the concentration of 20 μg/mL for 24 h. ROS production of MCF-7 cells stained by DCFH-DA after treatment with different samples for 4 h: J CLSM observation; K representative flow cytometric analysis. For D, E and I, data are means ± SD, n = 3, *P < 0.05; **P < 0.01; ***P < 0.005; ****P < 0.001