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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates

Fig. 5

Purification of a loaded protein cage assembled within E. coli cells via co-expression. a Encapsulin and mNeon are co-expressed in E. coli and a fraction of mNeonGreen is encapsulated upon interaction with encapsulin via a target peptide and self-assembly of encapsulin into a protein cage. b Upon cell lysis, the lysate contains encapsulin cages loaded with mNeon and excess mNeon outside the cage. c SDS-PAGE showing the successful purification of encapsulin (non-loaded) and encapsulin loaded with mNeon via MMC followed by TX-114 phase partitioning. d SDS-PAGE showing the successful purification of encapsulin (non-loaded) using anion exchange membrane adsorbers. L: load; MMC: multimodal chromatography; TX: Triton X-114 phase partitioning; FT: flow-through; E: eluate

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Journal of Nanobiotechnology

ISSN: 1477-3155

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