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Table 1 Buffer solvents used for CC700 purification

From: A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates

Buffer name

Composition

Usage

No salt/low pH

50 mM sodium phosphate; pH 6.5

Buffer exchange

No salt/high pH

50 mM sodium phosphate; pH 8.0

Buffer exchange

Low salt/low pH

50 mM sodium phosphate; 0.1 M sodium chloride; pH 6.5

Buffer exchange

Low salt/high pH

50 mM sodium phosphate; 0.1 M sodium chloride; pH 8.0

Lysis of cells

High salt/low pH

50 mM sodium phosphate; 1 M sodium chloride; pH 6.5

Buffer exchange

High salt/high pH

50 mM sodium phosphate; 1 M sodium chloride; pH 8.0

Buffer exchange

Ammonium sulfate/low pH

50 mM sodium phosphate; 1 M ammonium sulfate; pH 6.5

Buffer exchange

Ammonium sulfate/high pH

50 mM sodium phosphate; 1 M ammonium sulfate; pH 8.0

Buffer exchange