Table 1 Buffer solvents used for CC700 purification
Buffer name | Composition | Usage |
|---|---|---|
No salt/low pH | 50 mM sodium phosphate; pH 6.5 | Buffer exchange |
No salt/high pH | 50 mM sodium phosphate; pH 8.0 | Buffer exchange |
Low salt/low pH | 50 mM sodium phosphate; 0.1 M sodium chloride; pH 6.5 | Buffer exchange |
Low salt/high pH | 50 mM sodium phosphate; 0.1 M sodium chloride; pH 8.0 | Lysis of cells |
High salt/low pH | 50 mM sodium phosphate; 1 M sodium chloride; pH 6.5 | Buffer exchange |
High salt/high pH | 50 mM sodium phosphate; 1 M sodium chloride; pH 8.0 | Buffer exchange |
Ammonium sulfate/low pH | 50 mM sodium phosphate; 1 M ammonium sulfate; pH 6.5 | Buffer exchange |
Ammonium sulfate/high pH | 50 mM sodium phosphate; 1 M ammonium sulfate; pH 8.0 | Buffer exchange |