Fig. 4

SWCNTs-exposed MLE-12 cells caused FMT. MLE-12 cells were treated with 10 μg/ml SWCNTs for 6, 12 and 24 h, and then cell-free culture medium, as conditioned medium (CM), was used to culture primary lung fibroblasts for 48 h. A, B mRNA expressions of α-SMA and COLI in lung fibroblasts were measured by qRT-PCR (n = 4). C, D Western Blotting analysis of α-SMA protein expression in lung fibroblasts (n = 3). E COLI positive expression in lung fibroblasts was evaluated by IHC (n = 5) (200×). *P < 0.05 vs CTRL group