Fig. 3
Cellular protection and internalization. A Cell viability and B cell apoptosis of H2O2-induced RPE after treatments with different formulations. Data are shown as mean ± SD, n = 6. *P < 0.05, **P < 0.01 vs Model; #P < 0.05. Intracellular C C6 fluorescence and D RH&BCL after H2O2-induced RPE were treated with different formulations for 4 h. The bar is 100 μm. Data are shown as mean ± SD, n = 6. ***P < 0.001 vs C6; ###P < 0.001 vs C6-M. E Immunofluorescence staining of ZO-1 and ICAM-1 for untreated and H2O2-induced RPE. The bar is 40 μm. F CLSM images of H2O2-induced RPE treated with different C6-related formulations for 2 h. ZO-1 and formulations were labeled with red and green, respectively. The bar is 20 μm. G CLSM images of H2O2-induced RPE treated with different C6-related formulations for 2 h. ICAM-1 and formulations were labeled with red and green, respectively. The bar is 20 μm