Fig. 4
Anti-angiogenic studies in vitro. A Scratch tests of the formulations on HUVECs for 24 h and B corresponding wound recovery. The bar is 400 μm. C Tubule formation after HUVECs were treated with different formulations, and D corresponding total length, branch length, and the number of nodes. The bar is 100 μm. Data are shown as mean ± SD, n = 6. **P < 0.01, ***P < 0.001 vs Control; #P < 0.05, ##P < 0.01. E Investigations of migration and F invasion studied by Transwell. The bar is 100 μm. Data are shown as mean ± SD, n = 6. **P < 0.01, ***P < 0.001 vs Control; #P < 0.05. G ELISA tests of VEGF secreted by CoCl2-induced HUVECs treated with different formulations for 24 h. H Western blot analysis of HIF-1α expression of CoCl2-induced RPE. Data are shown as mean ± SD, n = 6. **P < 0.01 vs Model; #P < 0.05, ##P < 0.01