Fig. 4
From: Ratiometric electrochemical OR gate assay for NSCLC-derived exosomes
Ultrasensitive detections of exosomes. A Comparison of the biosensor with NanoFCM for exosome detection. B Determination of the selectivity of exosome detection against typical interfering substances, including 1 KU/mL of CA125, 1 mg/mL of NSE, Pro-GRP, SCCA, and CYF21-1, respectively. C DPV analysis of different concentrations of exosomes ranging 108–102 particles µL−1 (from a to g). D Linear calibration curve between the ratiometric intensities and logarithm of exosome concentrations. E DPV responses acquired from 5 working electrodes, under the constant level of exosomes (2 × 103 particles µL−1). F DPV responses acquired over two weeks, under the constant level of exosomes (107 particles µL−1). G Exosome expression were profiled in different types of NSCLC cell lines (A549, H460, H1299, H1975, H2030, and Calu-1 cells). H Linear correlation between ratiometric intensities IFc/IMB and A549 cell numbers ranging 103–107. I Ratiometric intensities of exosomes spiked into three clinical serum samples at final concentrations of 103/105 particles µL−1. Error bars referred to the standard derivation obtained from three independent experiments