Fig. 7

Effects of MEP1B on pTr2 cells in vitro. A–E MEP1B promotes pTr2 cell proliferation and migration. A The transfection efficiency of MEP1B overexpression was determined by PCR. B The cell viability of pTr2 cells was applied by CCK-8 assay. C EDU staining assay was performed to determine the cell proliferation changes after MEP1B overexpression. Scale bars = 100 µm. D Wound healing assay for the evaluation of migration of pTr2 cells. Scale bars = 500 µm. E Transwell migration assay revealed that MEP1B overexpression increased the cell numbers of migration. Scale bars = 200 µm. F–J Knockdown of MEP1B inhibits pTr2 cell proliferation and migration. F The transfection efficiency of MEP1B siRNA was determined by PCR. G The cell viability of pTr2 cells was applied by CCK-8 assay. H EDU staining assay was performed to determine cell proliferation changes after MEP1B knockdown. Scale bars = 100 µm. I Wound healing assay for the evaluation of migration of pTr2 cells. Scale bars = 500 µm. J Transwell migration assay indicated that knockdown MEP1B reduced the cell numbers of migration. Scale bars = 200 µm. CCK-8 cell counting kit-8. The data were presented as mean ± SD. *p < 0.05, **p < 0.01, and Student’s t-test