Fig. 3

ADNVs remold tumor microenvironment and reprogram TAM phenotypes. Mice were implanted with LLC and treated with ADNVs or vesicle as described in Fig. 2. Tumor tissues were collected at 21 d post inoculation. A Ranked analysis of differential gene expression. B Quantification of M1 (CD86⁺) and M2 (CD206⁺) populations by flow cytometry. **p < 0.01 (Student’s t-test). C Representative immunofluorescence staining for CD86 and CD206 at tumor sections (Scale bar = 100 μm). D qRT-PCR analysis of M1-marker genes (upper) and M2-related genes. *p < 0.05, **p < 0.01, ***p < 0.001 (Student’s t-test). E Quantification of CD4⁺, CD8⁺ and Granzyme B⁺ CD8⁺ cell populations in TME by flow cytometry. ns non-significant, *p < 0.05, ***p < 0.001 (Student’s t-test). The results are from one of three independent experiments. Shown are representative images, and the data are presented as means ± SEM