Table 1 The sequences of the primers
qRT‒PCR primer name | Primer sequence (5′-3′) |
|---|---|
Gapdh (forward) | TCCATGACAACTTTGGTATCG |
Gapdh (reserve) | TGTAGCCAAATTCGTTGTCA |
Alp (forward) | ATAACGAGATGCCACCAGAGG |
Alp (reserve) | TTCCACATCAGTTCTGTTCTTCG |
Runx2 (forward) | AGAATGGACGTGCCCCCTA |
Runx2 (reserve) | CTGGGGAAGCAGCAACACTA |
Col1 (forward) | CTGACTGGAAGAGCGGAGAG |
Col1 (reserve) | CGGCTGAGTAGGGAACACAC |
Ocn (forward) | TTCTGCTCACTCTGCTGACCC |
Ocn (reserve) | CTGATAGCTCGTCACAAGCAGG |
Uchl3 (forward) | AGCAATGCCTGTGGAACGAT |
Uchl3 (reserve) | TTTGGCTCTCTCTTCAGGGC |
Smad1 (forward) | ACCCCTACCACTATAAGCGAG |
Smad1 (reserve) | TGCTGGAAAGAGTCTGGGAAC |