Fig. 7
Efficient codelivery by AR-NADR of shRNA to suppress NOR1 expression in vitro. A Agarose gel electrophoresis assay of NA/shRNA complexes at different weight ratios. B Fluorescence images of GFP fluorescence in different treatments. C The detection of NOR1 mRNA levels by agarose gel electrophoresis assay and D NOR1 protein levels by western blot analysis upon different treatments. E The IC50 values of DDP in HCC cells in different treatment groups. F The cell efflux rates of DDP at different time points. (a) HepG2 cells (control), (b) HepG2/DDP cells (control), (c) Free shRNA, (d) Lipo8000-NC (Lipo8000-negative control plasmid), (e) Lipo8000-shRNA, (f) AR-NAR (AR-NA/shRNA). Scale bar: 50 μm. Data are presented as the means ± SD (n = 3) (intergroup comparisons: *p < 0.05, **p < 0.01, ****p < 0.0001)