Fig. 10

An experiment to determine overexpression was performed in vivo. A An experiment to determine overexpression was performed, where in SD rats were administered exosomes containing overexpressed miR-26b-3p via a tail vein injection. Intravital imaging revealed that fluorescence-labeled exosomes were highly concentrated in both lower limbs after 48 h of injection. Exosomes were labelled with miR-26b-3p (green) and nuclei were stained with DAPI (blue). B Immunofluorescence findings from tissue sections confirmed that exosomes and miR-26b-3p entered the growth plate chondrocytes. C In situ hybridization confirmed that miR-26b-3p was expressed in the proliferative regions of femoral growth plates in rats. D RT-qPCR analysis revealed that miR-26b-3p was highly expressed in growth plate chondrocytes. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test or three groups were compared using ANOVA followed by Tukey’s test. **P < 0.01vs. control