Fig. 5

The overexpression of miR-26b-3p suppressed the proliferation and bone formation of normal human chondrocytes. A miR-26b-3p was overexpressed in human chondrocytes. B CCK8 showed that upregulating miR-26b-3p inhibited proliferation of human chondrocytes. C Flow cytometric analysis revealed that cell cycle was arrested in the G0/G1 phase after the overexpression of miR-26b-3p. D, E Western blot and RT-qPCR identified the marker genes of chondrocyte hypertrophic differentiation, COL10A and RUNX2, showed obvious downregulation after the overexpression of miR-26b-3p. F, G Western blot and RT-qPCR observed that the osteogenic genes, OCN and OPN, were also downregulated following the overexpression of miR-26b-3p. H The activity of ALP was reduced in mineralization after the overexpression of miR-26b-3p. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control