Fig. 6

The exosomes with miR-26b-3p overexpression of chondrocyte suppressed the proliferation and bone formation. A Internalization of exosomes by chondrocyte examined by laser scanning confocal microscope. B miR-26b-3p was overexpressed in chondrocytes. C CCK8 showed that upregulating miR-26b-3p inhibited proliferation of chondrocytes after the overexpression of miR-26b-3p. D Flow cytometric analysis revealed that cell cycle was arrested in the G0/G1 phase after the overexpression of miR-26b-3p. E, F Western blot and RT-qPCR identified the marker genes of chondrocyte hypertrophic differentiation, COL10A and RUNX2, showed obvious downregulation after the overexpression of miR-26b-3p. G, H Western blot and RT-qPCR observed that the osteogenic genes, OCN and OPN, were also downregulated after the overexpression of miR-26b-3p. I The activity of ALP was reduced in mineralization after the overexpression of miR-26b-3p. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control