Fig. 7

miR-26b-3p in exosomes inhibits chondrocytes proliferation and endochondral ossification via the AKAP2 in vitro. A mRNA microarray assays was performed to identify differentially expressed genes after overexpression of miR-26b-3p. B The results showed that 316 mRNAs were differentially expressed, with 126 upregulated and 190 downregulated mRNAs. C, D Western blot and RT-qPCR analyses confirmed that AKAP2 expression was decreased upon overexpression of miR-26b-3p. E Luciferase activity assays revealed that co-transfection of AKAP2 with its mutant fragment restored luciferase activity, indicating that AKAP2 is a direct target of miR-26b-3p. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test. **P < 0.01, ***P < 0.001 vs. control