Fig. 8

Rescue experiments indicates that AKAP2 overexpression reversed the inhibition of human chondrocyte proliferation caused by overmiR-26b-3P. A AKAP2 presented downregulation after overexpression of miR-26b-3p, however, it was significantly improved in the group of miR-26b-3p upregulation + AKAP2. B, C Although CCK8 and cell cycle were inhibited in the miR-26b-3p mimic + NC group, whereas it was enhanced in the miR-26b-3p mimic + AKAP2 group. D–G Compared with the group of miR-26b-3p upregulation, the expression of marker genes of chondrocyte hypertrophic differentiation (COL10A and RUNX2) and the osteogenic genes (OCN and OPN) was also significantly improved in the group of miR-26b-3p upregulation + AKAP2 upregulation. H Although the activity of ALP in chondrocytes were suppressed after miR-26b-3p upregulation, it was reversed after AKAP2 was upregulated. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test or three groups were compared using ANOVA followed by Tukey’s test. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001 vs. control