Fig. 9

MiR-26b-3p inhibits estrogen receptor α (ER-α) via AKAP2/ERK1/2 axis in human chondrocytes. A, B The relative mRNA and protein expression levels of ER-α did not show statistical difference between the group of miR-26b-3p overexpression and control group. C The expression of p-ER-α was obviously downregulated in the group of miR-26b-3p overexpression compared with control group. D, E The expression of ERK1/2 protein and p-ERK1/2 protein. Similarly, significant differences on the relative mRNA and protein expression of ERK1/2 were not observed between the group of miR-26b-3p overexpression and control group in our study. F The expression of p-ERK1/2 protein was significantly decreased in the group of miR-26b-3p overexpression compared with control group. The data are presented as the mean ± SD. n = 3. Two groups were compared using T-test or three groups were compared using ANOVA followed by Tukey’s test. ns, not significant, **P < 0.01, ***P < 0.001 vs. control