Fig. 4

In vivo safety evaluations of the three kinds of the nanoparticles. A Schematic illustration of the experiments. Four groups of 8-week-old female BALB/c mice were injected with 100 μL PBS or PBS with bare NPs, with RBC-NPs or with MDCK-NPs (2% w/v) intravenously. B Survival curves of the mice in the next 7 days (n = 6). Five groups of 8-week-old female BALB/c mice, were injected intravenously with 100 μL PBS or PBS with nanoparticles (2% w/v) or N2a cell membrane (100 μL, approximately 5 × 10.⁷ cells). C Blood cell counts of the mice (n = 3). D Chemical composition analysis of serum (n = 3). E The concentration of IgE in plasma in the mice (n = 3). Data are presented as the mean ± SD. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p ≥ 0.05 (ns)