Fig. 8

In vivo evaluation of projection provided by preinjected RBC-NPs. A Schematic illustration of the experiments. Three treatment groups of mice were injected with 2 mg RBC-NPs intravenously 24, 48, and 72 h prior to injection with 20 ng GST-ETX intravenously. The negative and positive control groups of mice were injected with PBS intravenously in the 72 h prior to toxin challenge, and mice were injected with 20 ng GST-ETX or PBS intravenously on the last day. B Survival curves of the mice for 7 days post-intravenous toxin challenge (n = 6). C Schematic illustration of the experiments. Three treatment groups of mice were injected with 2 mg RBC-NPs via aerosol into the lungs at 24, 48, and 72 h prior to aerosol challenge with 50 ng GST-ETX. The negative and positive control groups of mice had PBS via aerosol in the 72 h prior to aerosol lung exposure with 20 ng GST-ETX or PBS on the last day. D Survival curves of the mice for 7 days after aerosol toxin challenge (n = 6). E Blood cell counts of cells of mice injected intravenously (n = 3). F Blood cell counts of mice injected via aerosol into lungs (n = 3). Data are presented as mean ± SD. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p ≥ 0.05 (ns)