Fig. 2

Biocompatibility and ROS scavenging properties in vitro. A CCK-8 assay was used to evaluate the cell cytotoxicity of SeNP and IGK@SeNP against NPCs after incubation for 24 h, 48 h, and 72 h. n = 6, mean ± SD. B, D Live/dead staining and cell viability of NPCs co-cultured with IGK@SeNP (60 μg/ml) for 24 h, 48 h, and 72 h. Scale bar = 200 μm. n = 3, mean ± SD. ns, not significant. C Fluorograph of the cellular uptake of Cy5@SeNP by NPCs. Scale bar = 100 μm. E, F Flow cytometry analysis and relative geometric mean fluorescence intensity of intracellular ROS levels in NPCs treated with H₂O₂ (200 μM) and different treatments. n = 3, mean ± SD. *p < 0.05 compared to the H₂O₂ group