Fig. 3
IGK@SeNP attenuates H2O2-induced ECM degradation. A Western blotting analysis of the expression of ECM anabolic proteins (COL2, SOX9, and ACAN) and ECM catabolic enzymes (MMP3, MMP13, and ADAMTS5) in 200 μM H2O2-induced NPCs treated with IGK (20 μM), SeNP (60 μg/ml), and IGK@SeNP (60 μg/ml). B Densitometric analysis of ECM metabolism-related protein levels. C–F IF staining of ACAN and MMP3 in NPCs. Scale bar = 100 μm. n = 3, mean ± SD. *p < 0.05 compared to the H2O2 group, †p < 0.05 compared to the IGK group