Fig. 4

IGK@SeNP inhibits H₂O₂-induced NPC apoptosis. A Western blotting analysis of the expression of an antiapoptotic protein (BCL2) and pro-apoptosis proteins (BAX and cleaved CASP3). B Densitometric analysis of apoptosis-related protein levels. C, D IF staining of cleaved CASP3 in NPCs. Scale bar = 100 μm. E, F TUNEL staining of NPCs after different treatments. Scale bar = 100 μm. G, H Flow cytometry analysis with FITC-annexin V/PI assay. *p < 0.05 compared to the H₂O₂ group, †p < 0.05 compared to the IGK group