Fig. 8

IGK@SeNP attenuates matrix degradation and cell apoptosis in vivo. A, B IHC staining of ACAN and MMP3 in rat coccygeal discs. C Quantitative analysis of ACAN- and MMP3-positive rates in different groups. D IF staining of cleaved CASP3 in rat coccygeal discs. E TUNEL staining of rat coccygeal discs. F Quantitative analysis of cleaved CASP3- and TUNEL-positive cells in different groups. Scale bar = 100 μm. n = 5, mean ± SD. *p < 0.05 compared to the IDD group, †p < 0.05 compared to the IGK group