Fig. 1

Isolation and characterization of OE-MSC-Exos and their suppression on Tfh cell responses in vitro. (a, b) Representative TEM (a) and SEM (b) images of exosomes derived from OE-MSCs. (c) The particle sizes of OE-MSC-Exos were determined by NTA. (d) Western blot analysis of CD63, CD9, TSG101, ALIX and Calnexin in OE-MSC-Exos. (e) The expression of typical identification markers of MSCs on the surface of OE-MSC-Exos. (f) Naïve CD4 + T cells from the spleen of C57BL/6 mice were cultured for 72 h in the presence of OE-MSC-Exos (30, 60, 90 µg/ml) under Tfh differentiation conditions and analyzed by flow cytometry assay. Values represent means ± S.D. (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001