Table 1 Cysteine mutagenesis within GvpA and effect on GVNPs formation
From: Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization
Variant | GVNPs phenotype | Diameter (µm) | Average (µm) | Width (µm) | Average (µm) | ||
|---|---|---|---|---|---|---|---|
min | max | min | max | ||||
GVNP (WT) | Spindle/cylinder | 0.26 | 0.83 | 0.438 | 0.11 | 0.27 | 0.207 |
1.S6C | Spindle/cylinder | 0.15 | 1.04 | 0.450 | 0.13 | 0.26 | 0.191 |
2.S7C | Spindle/cylinder | 0.24 | 1.35 | 0.557 | 0.13 | 0.27 | 0.179 |
3.S47C | Spindle/cylinder | 0.31 | 1.4 | 0.632 | 0.13 | 0.31 | 0.228 |
4.A2C | Negative | – | – | – | – | – | – |
5.A10C | Negative | – | – | – | – | – | – |
6.A45C | Spindle/cylinder | 0.24 | 1.61 | 0.532 | 0.17 | 0.29 | 0.224 |
7.A46C | Spindle/cylinder | 0.17 | 1.02 | 0.503 | 0.11 | 0.25 | 0.171 |
8.A64C | Mini spindle | 0.21 | 1.09 | 0.354 | 0.10 | 0.15 | 0.119 |
9.A68C | Spindle/cylinder | 0.38 | 2.03 | 0.733 | 0.15 | 0.28 | 0.227 |
10.A70C | Spindle/cylinder | 0.35 | 2.06 | 0.746 | 0.12 | 0.27 | 0.209 |
11.A72C | Spindle/cylinder | 0.34 | 1.31 | 0.730 | 0.14 | 0.33 | 0.243 |
12.A73C | Cylinder | 0.17 | 1.72 | 0.755 | 0.17 | 0.23 | 0.182 |
13.A76C | Cylinder | 0.23 | 2.04 | 1.095 | 0.14 | 0.18 | 0.162 |
14. S6C.A64C | Spindle/longer | 0.35 | 2.76 | 1.851 | 0.13 | 0.21 | 0.156 |
15. S6C.A68C | Cylinder/longer | 0.18 | 2.72 | 1.259 | 0.09 | 0.25 | 0.155 |
16. S6C.A70C | Spindle/cylinder/longer | 0.45 | 1.65 | 1.024 | 0.10 | 0.22 | 0.146 |
17. S6C.A72C | Spindle/cylinder/longer | 0.37 | 1.86 | 0.972 | 0.14 | 0.25 | 0.188 |
18. S6C.A73C | Cylinder/longer | 0.19 | 2.36 | 0.974 | 0.10 | 0.21 | 0.142 |
19. S6C.A76C | Cylinder/longer | 0.49 | 1.73 | 1.049 | 0.13 | 0.21 | 0.166 |
20. S7C.A64C | Negative | – | – | – | – | – | – |
21. S7C.A68C | Spindle/cylinder/longer | 0.26 | 1.91 | 0.682 | 0.09 | 0.25 | 0.175 |
22. S7C.A70C | Spindle/cylinder | 0.36 | 1.54 | 0.890 | 0.08 | 0.19 | 0.138 |
23. S7C.A72C | Spindle/cylinder/longer | 0.14 | 2.04 | 0.711 | 0.08 | 0.19 | 0.151 |
24. S7C.A73C | Cylinder/longer | 0.32 | 1.88 | 1.028 | 0.09 | 0.24 | 0.158 |
25. S7C.A76C | Negative | – | – | - | – | – | – |
26. S6C.A64C.A70C | Spindle/longer | 0.22 | 2.36 | 1.015 | 0.05 | 0.19 | 0.125 |
27. S7C.A64C.A70C | Negative | – | – | – | – | – | – |
28. S6C.A64C.A72C | Spindle/longer | 0.29 | 2.73 | 1.226 | 0.10 | 0.21 | 0.171 |
29. S7C.A64C.A72C | Negative | – | – | – | – | – | – |
30. S6C.A64C.A73C | Spindle/cylinder/longer | 0.24 | 1.38 | 0.759 | 0.11 | 0.23 | 0.162 |
31. S7C.A64C.A73C | Negative | – | – | – | – | – | – |
32. S6C.A64C.A76C | Spindle/longer | 0.23 | 2.33 | 1.119 | 0.06 | 0.17 | 0.104 |
33. S7C.A64C.A76C | Negative | – | – | - | – | – | – |
C.GvpA (N-ter) | Negative | – | – | - | – | – | – |
GvpA.C (C-ter) | Spindle/cylinder | 0.18 | 0.80 | 0.444 | 0.10 | 0.23 | 0.157 |