Fig. 4
a Intracellular ROS images of 4T1 cells after treated with PBS, HFNP, HFNP@GOX & HFNP@GOX@PFC for 24 h. b The expression of Glut-1, HIF-1α and CD47 in 4T1 cells examined by Western blotting, c corresponding quantitative analysis and d CD47 FCM after treatment with PBS, HFNP, HFNP@GOX & HFNP@GOX@PFC for 24 h. e Apoptosis and f corresponding quantitate analysis of 4T1 cells after above treatments, detected by FCM. g Bcl-2, BAX, CytC, Casp3 and p53 relative protein levels and h corresponding quantitative analysis of 4T1 cells treated as above, detected by Western blotting. Scale bar: 200 μm. Error bars present as mean ± SD (n = 6)