Fig. 5

a Schematic diagram of tumor cells and macrophages co-culture assay via Transwell. b The percentage of CD86 positive cells and c corresponding quantitate analysis. 4T1 cells seeded on the lower station were cultured with above treatment groups for 24 h. RAW264.7 cells were co-stained with PerCP-Cy5.5-CD11b, AF488-F4/80 and PE-CD86, followed by FCM analysis. d TNF-α, IL-6 and IL-10 cytokines concentration of supernatant in 4T1 and RAW 264.7 cells co-culture system, detected by ELISA. e The expression of SYK, PLCγ2, ASK1, pp38 and p38 in RAW264.7 cells examined by Western blotting and f corresponding quantitative analysis using Image J software. g Schematic diagram and results h of wound healing, migration and invasion assays of 4T1 cells via co-culture system. Scale bar: 100 μm. Error bars present as mean ± SD (n = 6)