Fig. 3
The efficiently induce tumor cell death by P-IDI. a Confocal images of the 4T1 cells after incubating with P-IDI (100 μg IDI/mL) for 0.5, 1, 2, 4, and 6 h (Blue: Hoechst 33342; Red: Cy5.5). Scale bar: 50 µm. b Flow cytometry analysis of internalization of P-IDI. c Representative bio-TEM images of 4T1 cells incubated with P-IDI (100 μg IDI/mL) for 2 and 24 h. Scale bars were 2 µm, 1 µm and 200 nm from left to right, respectively. d Hemolysis analysis of P-IDI (25–500 μg IDI/mL) solutions at various concentrations (mean ± SD, n = 3). The mixtures after kept standing for 3 h were centrifuged to detect the hemoglobin in the supernatant visually (inset). e Cell viabilities of 4T1 cells measured by MTT assays, after incubating with different concentration of P-IDI with or without 1064 nm laser (1 W/cm2, 5 min) irradiation. f Fluorescence images of Calcein AM (live cells, green fluorescence) and propidium iodide (PI) (dead cells, red fluorescence) co-stained 4T1 cells with different treatments, including control and P-IDI (100 μg IDI/mL) for 12 h with or without laser irradiation (1064 nm, 1 W/cm2, 5 min). Scale bar: 100 µm