Fig. 5
ICD induced by P-IDI based PTT. a Representative fluorescence images of 4T1 cells showing CRT exposure on the surface after various treatment (100 μg IDI/mL, Laser: 1064 nm, 1 W/cm2 for 5 min). Scale bar: 10 μm. b ATP release after various treatments. c HMGB1 secretion in the medium after various treatments. d–f TNF-α, IL-6, IL-10 of DCs after incubation with 4T1 cells treated by P-IDI (100 μg IDI/mL) with laser irradiation (1 W/cm2, 5 min). g, h Flow cytometric analyses of the populations of matured DC cells and CD8+ T cells in splenocytes of mice immunized after the different treatment. Statistical significance was assessed via unpaired two-sided Student t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 versus control