Fig. 6

Subcutaneous tumor imaging in vivo. (A) NIR fluorescence images of subcutaneous 4T1 and MS751 xenograft model mice at different time intervals post-injection (0.5 h, 2 h, 4 h, 12 h, 24 h, 72 h). TMVP1-ICG-NPs and ICG-NPs were injected via the tail vein at a dose of 1.0 mg/kg. The red circle showed the tumor site with fluorescence. (B) Representative NIR fluorescence images of the isolated tumors and organs at 24 h post-injection (T, tumor; H, heart; Li, liver; S, spleen; Lu, lung; K, kidney; I, intestine). (C) The mean NIR fluorescence signal intensity of 4T1 subcutaneous tumors was measured using the ROI tool at different time points in the two groups. (D) The mean NIR fluorescence signal intensity of the 4T1 subcutaneous tumor and organs from mice of the two groups at 24 h. (E) The mean NIR fluorescence signal intensity of MS751 subcutaneous tumors was measured using the ROI tool at different time points in the two groups. (F) The mean NIR fluorescence signal intensity of the MS751 subcutaneous tumor and organs from mice of the two groups at 24 h. Values of p < 0.05 were considered significant (ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)