Fig. 7

Lymph node metastasis foci imaging in vivo. (A-B) NIR fluorescence images of 4T1/MS751 footpad lymph node metastasis model mice and normal mice at different time points (10 min, 2 h, 4 h, 8 h, 12 h, and 24 h). The right footpad was injected with TMVP1-ICG-NPs and the left footpad was injected with ICG-NPs. (C-F) The mean NIR fluorescence signal intensity of the lymph nodes with tumor metastasis and normal lymph nodes at the different time points after bilateral injection of the corresponding drugs. (G) Representative NIR fluorescence images of isolated lymph nodes with tumor metastasis and normal lymph nodes at 4 h post-injection. (H-I) The mean NIR fluorescence signal intensity of popliteal lymph nodes of (G) was measured using the ROI tool. Values of p < 0.05 were considered significant ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)