Fig. 8

UCMSCs-exo/eNOS inhibit the inflammatory response associated with chronic trauma in diabetic mice. I: Treatment with PBS. II: Treatment with UCMSCs-exo. III: Treatment with UCMSCs-exo/eNOS. (a) Immunofluorescence staining of Ly6G in skin wounds of PBS, UCMSCs-exo, and UCMSCs-exo/eNOS-treated diabetic mice on postoperative day 7. Scale bar: 100 μm. (b) Immunofluorescence quantification of Ly6G (n = 6 in each group, two-tailed Student’s t-test). (c) RT-qPCR analyis of the expression of inflammation-associated cytokines in the wound tissue on treatment day 14 (at least three independent replicate experiments). (d) Immunohistochemistry of inflammation-associated cytokines (IL1β, IL6, and TNF-α) in wound tissue on treatment day 14. (e) (f) (g) Quantification of immunohistochemical staining of IL-1β, IL6, and TNF-α (n = 6 in each group, two-tailed Student’s t-test). Data represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 vs. PBS group; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. UCMSCs-exo group