Fig. 1
CD133+ Huh7 cell population contributes to sorafenib resistance. A, B CD133 positive Huh7 cells were sorted and verified with Flowcytometry. C The expression of liver cancer stem cell markers (ALDH/ EpCAM/ CD90) was examined in sorted and unsorted Huh7 cells by western blotting. D The expression of CSCs markers (CD133/EpCAM/ ALDH/ CD90) was analyzed using immunostaining. Scale bar: 1 µm. E Different concentration of sorafenib was used to treat CD133+ Huh7, CD133− Huh7 and Huh7 cells, the IC50 value was analyzed with CCK-8 assay. Huh7 cells was set as control. Data are expressed as mean ± SD. n = 3; ***p < 0.001 by student’s t-test