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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Engineered extracellular vesicles mediated CRISPR-induced deficiency of IQGAP1/FOXM1 reverses sorafenib resistance in HCC by suppressing cancer stem cells

Fig. 2

Characterization of engineered EVs. A Schematic diagram for isolation procedure of engineered EVs. B TEM, C DLS and D NTA analysis showed the morphology, size distribution and particle concentration of engineered LC9-EVs and HLC9-EVs. E Western blotting analysis of CD40, Flag and AcGFP (red arrows) in both engineered cells and its produces EVs. CD63 and ANXA5 have been used to exclude the presence of exosomes (small-sized EVs) and apoptotic antibodies (large-sized EVs) while Cytochrome C (Cyt C) has been used as the negative marker of EVs

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Journal of Nanobiotechnology

ISSN: 1477-3155

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