Fig. 3

EVs-mediated liver cancer cell specific targeting. A Western blotting analysis of the presence of GPC3 expression in Huh-7 and LO2 cells. B In co-culture model, GPC3-mediated liver cancer cell specific targeting of EVs at 3 h post treatment. Red represents GPC3⁺ mcherryHuh-7 cells. Green represents LC9-EVs and HLC9-EVs. Scale bars: 1 µm. C, D FACS analysis of co-cultured cells post EVs treatment and quantitative evaluation respectively. LC9-EVs was used as control. Data are expressed as mean ± SD. n = 3; ***p < 0.001 by student’s t-test. E In vivo biodistribution of DiD-labeled EVs administrated intravenously in Huh7 xenograft mice and F corresponding fluorescent intensity quantification at tumor sites. LC9-EVs was used as control. Data are expressed as mean ± SD. n = 3; *p < 0.05 by student’s t-test