Fig. 5

Combined therapy of EVs -mediated IQGAP1/FOXM1 destruction with sorafenib. A–C CCK-8 analysis of CD133⁺ Huh7, CD133⁻ Huh7 and Huh7 cells.10 μM concentration of sorafenib was used. n = 5. D–F Corresponding apoptotic rate was evaluated with FACS by using Annexin V/PI staining kit. D Differential protein expression level of target gene IQGAP1/FOXM1 along with apoptosis markers (pro-Caspase3, BCL2 and BAX) were examined. GAPDH was used as an internal control. Mock group was set as control, data are expressed as mean ± SD. n = 3; *p < 0.05, ***p < 0.001, by student’s t-test