Fig. 3
From: Evolocumab loaded Bio-Liposomes for efficient atherosclerosis therapy
Cellular uptake of M@Lipo NPs. (A) Representative photos of Lipo/M@Lipo (red), HUVECs (blue), and ICAM-1 (green) in the LPS-treated group. (B) Semi-quantitative analysis of the uptake of different NPs in VSMCs and HUVECs. Data are means ± SD, n = 3, ***P < 0.001 vs. Lipo. (C) The schematic diagram showed that HUVECs were co-cultured with VSMCs in a transwell system for simulating plaque in vitro. (D&E) Phagocytosis of Lipo@Rho NPs and M@Lipo@Rho NPs in VSMCs and HUVECs in transwell. BF indicates bright field. (F) Fluorescence quantitation of Lipo/M@Lipo (red). Fluorescence images (G) and quantitation (H) of VSMCs 4 h after incubation with different concentrations of M@Lipo NPs. Scale bars = 60 μm