Fig. 3

A The cell viability of 4T1 cells after treatment with different concentrations of Au@FePt for 24 h and 48 h (n = 5). B Cell viability test of Au@FePt and with and without X-ray or laser irradiation for 24 h (n = 5). Data represent mean ± SD from three independent experiments, each performed in five replicates (*P < 0.05, **P < 0.01). C Flow cytometry analyses of cell apoptosis by Annexin V-FITC/PI co-staining of different treatment groups. D Calcein-AM/PI co-staining of 4T1 cells after the indicated treatments (green: live cells; red: dead cells) of various treated groups (scale bar = 100 μm)