Fig. 5
A Fe2+ fluorescence staining without and with FePt nanoprobes treatment in 4T1 cells (scale bar: 50 μm). B Fluorescence images of lipid peroxide in 4T1 cells after treatment with PBS, FePt-Fer-1, Au@FePt + NIR, and Au@FePt + NIR + X-ray (scale bar: 50 μm). C Intracellular ROS generation was detected by the DCFH-DA probe after various treatments in 4T1 cells (Fe concentration: 5 mM, X-ray: 4 Gy) (scale bar: 50 μm) (*P < 0.05, **P < 0.01). D Western blot analysis of apoptosis-associated proteins after 48 h treatment within different nanoprobe-incubated cells. E, F Quantitative measurement of apoptosis-associated proteins in different nanoprobe-incubated cells during western blot imaging process (n = 3)