Fig. 2

TNTs inhibited Lamin A/C-regulated inflammation. (A) Schematic illustration of the modulation of lamin A/C expression to regulate macrophage inflammation. B, C) Western blotting analysis of lamin A/C expression in macrophages cultured on pTi and treated with Lmna siRNA (si-Lmna) or nontargeting siRNA (si-N) for 24 h (B) and quantitative analysis (C). (D) Relative expression of the inflammatory genes IL-1β, IL-6 and CXCL9 in macrophages cultured on pTi after lamin A/C knockdown and stimulation with LPS for 6 h. E, F) Western blotting analysis of lamin A/C expression in lamin A/C-overexpressing RAW264.7 or blank lentiviral vector-expressing control cells cultured on TNTs (E) and quantitative analysis (F). G) Relative expression of the inflammatory genes IL-1β, IL-6 and CXCL9 in macrophages shown in (E) and stimulated with LPS. The data are presented as the mean ± SD of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001