Fig. 4

Emerin was required for the modulation of inflammation by Lamin A/C. A) Representative images of emerin in macrophages cultured on pTi and TNTs and stimulated with LPS for 6 h. Right: normalized fluorescence intensity profiles of lamin A/C (red), emerin (green) and DAPI (blue) along the yellow line crossing cells are shown. Scale bar, 5 μm. B-C) Effects of emerin siRNA (si-emerin) or negative control siRNA (si-N) transfection on RAW264.7 cells cultured on pTi were analysed by western blotting. D-E) Representative images of the F/G-actin ratio in emerin siRNA-transfected macrophages cultured on pTi and stimulated with LPS. F) Representative images of emerin in lamin A/C-overexpressing macrophages cultured on TNTs after 6 h of LPS stimulation. Representative images of F/G-actin (G-H) and inflammatory gene expression (I) in lamin A/C-overexpressing macrophages and si-emerin + lamin A/C-overexpressing macrophages cultured on TNTs after LPS stimulation. The data for analysing the F/G-actin ratio were pooled from 28–40 single cells in each group from at least 6–10 randomly chosen fields. Western blotting and qRT‒PCR data are presented as the mean ± SD of three biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001