Fig. 5

TNTs regulated lamin A/C levels via actomyosin contractility. A-C) Representative fluorescence images with corresponding 3D surface plot images (A) and Western blotting analysis (B, C) of phosphorylated myosin light chain (pMLC) levels in macrophages cultured on pTi and TNTs and stimulated with LPS for 6 h. D, E) Western blotting analysis of pMLC and lamin A/C levels in RAW264.7 cells cultured on pTi and TNTs for 1, 2, 4, and 24 h. F, G) Western blotting analysis of lamin A/C in macrophages cultured on pTi and treated with blebbistatin (Bleb.). H, I) Under LPS stimulation, the levels of lamin A/C in macrophages cultured on pTi and treated with blebbistatin. The data are presented as the mean ± SD of three biological replicates. * When comparing TNTs to pTi or # when comparing 4 or 24 h versus 1 h of adhesion, *p < 0.05, **p < 0.01, ***p < 0.001