Fig. 2

The effect of MCMD NPs on cell viability, DC maturation, and activation. The cell viability of A HUVECs and B E.G7-OVA treated with free DOX or MCMD NPs. C Quantitative analysis of average fluorescence intensity of CpG in DCs. D CLSM images of DCs after co-incubation with free CpG or MC NPs. Red: lysosome. Green: Cy5-labeled CpG. Blue: nucleus. The cytokine secretion levels of E IFN-α, F IFN-β, and G TNF-α were measured with ELISA assays. The expression levels of H MHC-I, I CD86, and J CD40 were measured with flow cytometers. Data were expressed in the form of mean ± SD (n = 5). * P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001