Fig. 4

MCMD NPs significantly enhanced the antitumor immune responses in vivo. Representative FACS plots and histogram of percentage of CD3⁺ CD4⁺ and CD3⁺ CD8⁺ T cells in A–D tumors (A, B: CD3⁺CD4⁺; C, D: CD3⁺CD8⁺) and E–H spleens (E, F: CD3⁺CD4⁺; G, H: CD3⁺CD8⁺). Cytokines I IL-1β, J IL-12, K IFN-α, and L IFN-γ were evaluated with ELISA kits. Data were expressed in the form of mean ± SD (n = 5). * P < 0.05, ** P < 0.01, ***P < 0.001, ****P < 0.0001