Table 1 List of Primer Sequences for Real-Time PCR
From: Nano hydrogel-based oxygen-releasing stem cell transplantation system for treating diabetic foot
Primer name | Primer sequence (5’→3’) |
|---|---|
GAPDH-F | GGAGCGAGATCCCTCCAAAAT |
GAPDH-R | GGCTGTTGTCATACTTCTCATGG |
bFGF-F | GCAGAAGAGAGAGGAGTTGT |
bFGF-R | ACCGGTAAGTATTGTAGTTA |
IGF-1-F | TATGGCTCCAGCAGTCGGAGG |
IGF-1-R | TGGGTCTTGGGCATGTCGGT |
HGF-F | ATGATGTCCACGGAAGAGGA |
HGF-R | TTGTGCATCCATAATTAGGT |
VEGF-F | TACCCTGATGAGATCGAGT |
VEGF-R | CTCCTATGTGCTGGCCTTGGT |
PDGF -F | AGATTCCTCGGAGTCAGGTCG |
PDGF-R | ACGTATTCCACCTTGGCCAC |
ANGPT1 -F | TATTCACAGTATGACAGATT |
ANGPT1-R | GCACATTTGCACATACAGT |
IL-6-F | CCACTTCACAAGTCGGAGGCTTA |
IL-6-R | GCAAGTGCATCATCGTTGTTCATAC |
α-SMA-F | TGACCCAGATTATGTTTGAGACC |
α-SMA-R | CCAGAGTCCAGCACAATACCA |
CD31-F | TCATTGCGGTGGTTGTCATT |
CD31-R | TTGGCTTCCACACTAGGCTCAG |
Collagen I-F | AGGCCACGCATGAGCCGAAG |
Collagen I-R | GCCATGCGTCAGGAGGCAG |
Collagen III -F | AGGATCTGAGGGCTCGCCAGG |
Collagen III -R | AGCCACCAGACTTTTCACCTCCA |
CD80-F | TATGGAGATGCTCACGTGT |
CD80-R | TTCTGTGCTTACAGAAGCA |
CD206-F | CGAGACTGCTGCTGAGTCC |
CD206-R | GTACGAAGAACAGTGGATA |
Arg-1-F | TCTCTACATCACAGAAG |
Arg-1-R | GTTCACAGTACTCTTCA |