Fig. 3

Analysis of the immune response in liposome-vaccinated mice. A Schematic representation of the treatment schedule (immunization and sample collection). Female C57BL/6 mice were immunized s.c. at Days 1, 7, 14, 28 and 35. Sera were collected at Days 20, 34, 39 and 42 after vaccination. B Antibody titers of Day 39 sera. 4S was used as the coating antigen. Each data reflects an average of three measurements using pooled sera from each of the six groups (Day 39) and goat anti-mouse Ig(G + M). Error bars represent the standard error. C Antibody titers elicited by NLP-4S and NLP-4S-PBS57. 4S was used as the coating antigen. Each line represents the average of sera from Days 20, 34, 39 and 42 from 5 replicate mice (each dot) and goat anti-mouse Ig(G + M). Statistical differences were determined by two-tailed unpaired t test analysis and is indicated as either non-significant (ns) or *0.01 < P < 0.05