Fig. 4

Analysis of the subtype and specificity of antibody. A Analysis of the antibody subtype (IgG and IgM). 4S was used as the coating antigen. Each data reflects the ranges of three measurements using pooled sera from the NLP-4S and NLP-4S-PBS57 groups (Day 39) and goat anti-mouse Ig(G + M), IgG or IgM. The lines represent the mean values. B Analysis of antibody specificity. Disaccharide (GlcNAc2-PP-Phy), trisaccharide (GalGlcNAc2-PP-Phy), 4S (SiaGalGlcNAc2-PP-Phy) and biantennary complex type N-glycan (SiaGalGlcNAc)2Man3GlcNAc2-AsnFmoc were used as the coating antigens respectively. Each data reflects an average of three measurements using pooled sera from the NLP-4S-PBS57 (Day 39) and goat anti-mouse IgG. Error bars represent the standard error. Statistical differences were determined by two-tailed unpaired t test analysis and is indicated as non-significant (ns), *0.01 < P < 0.05 or **0.001 < P ≤ 0.01