Fig. 3

PSLipos-L-NAC had a stronger anti-inflammatory effect on M1 macrophages. The mRNA expression levels of pro-inflammatory cytokines of M1 macrophages treated with NAC, PSLipos and PSLipos-NAC respectively were analyzed by real-time quantitative PCR. (A) TNF-α, (B) IL-1β, (C) IL-6, (D) iNOS. (E) ROS production in M1 macrophages was evaluated by DCFH-DA. ns represented no significant difference, *p < 0.05, **p < 0.01, ***p < 0.001. The results represented the mean values ± SD. (n = 3)